Some enemies could be small gremlins playing trumpets, medium sized gremlins playing some french trumpets, which would require twice the effort to defeat than the normal ones, and some large room blocking robots playing smaller tubas than the tuba king. The large room blocking ones could have items required to beat them, it just take a hell of a beating before it goes down. Similar to Unicorn I’M A Nurse I Wear A Bikini I Drink And I Know Things Shirt the hotdogs (yes they are called hotdogs) from mega man. You play more stages, it builds itself closer and closer to the tuba king we all know and love.
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How about he has to collect each tuba from a “tuba lord” of sorts. There would be a bunch of these tuba lords, each with one tuba. He gets progressively stronger as he collects each tuba. Maybe the first tuba would be given to him for free by a lord that defected. These tuba lords can use the Unicorn I’M A Nurse I Wear A Bikini I Drink And I Know Things Shirt tubas against him with a variety of sound based attacks, and a move at low health could be just slamming the tuba around. The kind has to try and retrieve the tuba in as good a condition as possible. First stage should be a normal tuba as like there’s just a tuba on the floor and it turns into 1 but of the tuba king, and as.